About the Position The Department of Lab Medicine and Pathology, at the University of Minnesota invites applications for a full-time Researcher 3 position to support human gene replacement mouse modeling research for Alzheimer’s disease and Alzheimer’s disease related dementias. The successful candidate will assist with reproductive technology procedures in mice for creating a variety of mouse transgenic models. A successful candidate will take a new perspective using existing solutions, work independently with minimal guidance, and will come to the position with working knowledge of the field of work.
Responsibilities: • 35% Participates in generating chimeric mice from targeted mES cell by injection into blastocysts. • 25% Participates in mouse embryonic stem cell culture and gene targeting. • 20% Conduct genome editing in mice using CRISPR/Cas9 microinjection of zygotes. • 15% BAC modification and plasmid construct design. • 2.5% Maintain a clean and orderly work environment • 2.5% Follow established biological, chemical and physical safety procedures
The University of Minnesota offers a comprehensive benefits package including:
· Competitive wages, paid holidays, vacation and sick leave
· Low cost medical, dental, and pharmacy plans
· Health care and dependent daycare flexible spending accounts
· Excellent retirement plans with employer match
· Disability and employer paid life insurance
· Wellbeing program with reduced insurance premiums
· Tuition reimbursement opportunities covering 75%-100% of eligible tuition
· Student loan forgiveness opportunity
· Opportunities for growth and promotion
· Employee Assistance Program
For more information regarding benefits: https://humanresources.umn.edu/sites/humanresources.umn.edu/files/2021_tcd-cslr-75100-ben-0105-7_final.pdf
All required qualifications must be documented on application materials.
Required Qualifications • BS/BA in Molecular Biology, Biology or related field and 2 years of related experience or a combination of education and experience to equal 6 years • Must have experience in mouse handling and willingness to learn assisted reproductive techniques. • Must have aseptic techniques and cell culture experience. • Hands-on experience with molecular biology procedures (DNA isolation, PCR, gel electrophoresis) • Ability to work well within the lab group and with collaborators.
Preferred Qualifications • Experience with various assisted reproductive techniques in the mouse to include superovulation, IVF, preparing pseudo pregnant recipients, surgical transfer of embryos and sperm cryopreservation. • mESC culture experience. • DNA cloning experience. • Excellent organizational and oral/written communication skills • Excellent problem solving skills
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.