About the Position The Department of Lab Medicine and Pathology, at the University of Minnesota invites applications for a full-time Researcher 4 position to support human gene replacement mouse modeling research for Alzheimer’s disease and Alzheimer’s disease related dementias. The successful candidate will use assisted reproductive technologies in mice for creating a variety of mouse transgenic models. A successful candidate will be able to interpret internal and external issues and recommends solutions and/or best practices, solve complex and varied problems, and experience taking broad disciplinary perspective to identify solutions.
90% Technical laboratory duties
20% Mouse embryonic stem cell culture and gene targeting.
35% Generation of chimeric mice by microinjection of targeted mESC’s into blastocysts.
20% Genome editing in mice using CRISPR/Cas9 microinjection of zygotes.
15% BAC modification and plasmid construct design.
10% General laboratories duties
2.5% Contribute to manuscript preparation and & review literature to incorporate into the manuscripts as background or discussion
2.5% Discuss possible troubleshooting techniques based on literature review or meetings with other colleagues
2.5% Maintain a clean and orderly work environment
2.5% Follow established biological, chemical and physical safety procedures
For HR Use Only:
The University of Minnesota offers a comprehensive benefits package including:
· Competitive wages, paid holidays, vacation and sick leave
· Low cost medical, dental, and pharmacy plans
· Health care and dependent daycare flexible spending accounts
· Excellent retirement plans with employer match
· Disability and employer paid life insurance
· Wellbeing program with reduced insurance premiums
· Tuition reimbursement opportunities covering 75%-100% of eligible tuition
· Student loan forgiveness opportunity
· Opportunities for growth and promotion
· Employee Assistance Program
For more information regarding benefits: https://humanresources.umn.edu/sites/humanresources.umn.edu/files/2021_tcd-cslr-75100-ben-0105-7_final.pdf
All required qualifications must be documented on application materials.
Required Qualifications • Master’s degree in Molecular Biology, Biology or related field and 2 years of related experience or BS/BA and 4 years of related experience • Must have experience in mouse handling and assisted reproductive techniques. • Must have aseptic techniques and cell culture experience (Transfection, selection, expansion, cryopreservation). • Hands-on experience with molecular biology procedures (DNA isolation, PCR, gel electrophoresis, transformation). • Ability to work well within the lab group and with collaborators. • Evidence of being capable of troubleshooting experimental problems independently and to think critically about their research.
Preferred Qualifications • Experience with various assisted reproductive techniques in the mouse to include superovulation, IVF, preparing pseudo pregnant recipients, surgical transfer of embryos and sperm cryopreservation. • mESC culture experience. • DNA cloning experience. • Excellent organizational and oral/written communication skills
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.