A highly qualified and motivated research technician, with a minimum of three years of experience working in a research lab, is sought to join the Ankeny laboratory in the Department of Surgery, Division of Surgical Oncology at the University of Minnesota in Minneapolis, MN. This laboratory will focus on understanding the tumor immune microenvironment of pancreatic malignancies from the standpoint of dendritic cell subsets, phenotypes, and their roles in activating or inhibiting anti-tumor immune cells. We will also seek to understand the role that local and systemic immune therapies play in perturbing this system for the achievement of treatment benefit in patients with these cancers. To answer these fundamental questions, we will rely heavily on analysis of both genetically engineered mouse models and transgenic mice, and also fresh human tissues. We will utilize tissue processing methods, advanced flow cytometry, RNAseq, single cell RNAseq, immune cell isolation, culture, and expansion, and multiple immune assays to understand immune dysfunction, at the level of antigen presenting cells, throughout the development and progression of pancreatic cancer. The lab will share space with the Provenzano lab (another lab working on pancreatic cancer), but the technician will report to Dr. Ankeny. This represents an excellent opportunity for a highly motivated individual to work and learn in a tight-knit team and directly contribute to the study of relevant problems in cancer.
Independent Conduct of Scientific Experiments (80%) • Maintenance and treatment of cells in culture as needed. • Care of KPC genetic or related mice in facilities. • Establishment of genetic tumor mouse models, treatment of animals, and assessment of treatment response via viability assays, immune assays, and cytokine profiling. • Perform mouse necropsy to analyze mouse tissues for immune cell populations, phenotypes, and underlying mechanisms. • Processing and gradient centrifugation of peripheral blood samples. • Processing and dissociation of fresh tissue specimens. • Preparation and processing of single cell suspensions for multi-color flow cytometry. • Preparation and processing of fresh tissue for immunofluorescence or histocytometry. • Preparation and processing of single cell suspensions or fresh tissue for genetic analysis including bulk RNAseq, single cell RNAseq, and possible exome sequencing or PCR. • Perform routine molecular biology analyses as needed. • Evaluate the effects of therapeutics on cells, tissue samples, and in animal models as above. • Develop and troubleshoot new techniques and assays. • Develop new protocols and ensure they are followed precisely. • Independently problem solve/resolve issues with scientific experiments and recommend solutions.
Laboratory Management (10%) • Maintain laboratory safety by ensuring that the lab is compliant with appropriate safety regulations and that lab specific protocols are established and followed. • Ensure compliance with record keeping and safety training. • Oversee the tracking and restocking of lab supplies. • Perform validation exercises for all aspects of reagent and supply preparation and processing. • Other maintenance and supply duties as required.
Data Management (5%) • Analyze, summarize, and report experimental data to Principal Investigator as well as other members of the research team as needed. • Oversee meticulous record keeping of all experiments and projects. • Other data management duties as required.
Literature review, R&D, and additional duties (5%) • Review relevant research literature to facilitate job performance. • Attendance at weekly lab meetings. • Collaborate on the preparation of protocols, presentations, and manuscripts. • Oversee ongoing R&D operations as time allows and as assigned by Principal Investigator. • Other duties as required.
The University of Minnesota offers a comprehensive benefits package including:
Competitive wages, paid holidays, vacation and sick leave
Low cost medical, dental, and pharmacy plans
Health care and dependent daycare flexible spending accounts
Excellent retirement plans with employer match
Disability and employer paid life insurance
Wellbeing program with reduced insurance premiums
Tuition reimbursement opportunities covering 75%-100% of eligible tuition
All required qualifications must be documented on application materials.
Required Qualifications: • Masters degree and 3 years of related experience or BA/BS and at least 4 years of experience working in a biological lab. • Experience in immunology, molecular biology, and cell biology or related field. • Ability to work independently in a rapidly changing scientific environment and conduct experiments independently. • Strong written and verbal communication skills. • Analytical skills to process complex and varied scientific problems and identify solutions. • Demonstrated attention to detail and ability to follow directions/protocols. • Meticulous organizational and time management skills. • Experience with Microsoft Excel and PowerPoint or similar programs for data management and presentation creation. • Must be able to stand at a laboratory bench or hood for extended periods of time and lift up to 40 lbs. • Must be able to work flexible hours as needed by project. • Must be able to work on site in a laboratory setting
Preferred Qualifications: • Graduate level coursework. • Previous experience working in a BSL2 laboratory setting. • Prior experience with blood processing, centrifugation, and tissue dissociation. • Prior experience with multi-color advanced flow cytometry. • Prior experience with single cell RNAseq. • Prior experience with immune cell culture and basic immunoassays. • Prior experience with tissue immunohistochemistry or histocytometry. • Prior experience with animal studies and care using mice. • Experience using the following equipment is preferred: fluorometer, spectrophotometer, spectrometer, centrifuge, speed-vac, microscopes, biological safety cabinet, autoclave, incubators, manual pipet, electronic pipet, digital balance, centrifuges, lab chillers and water baths, fume hoods, gas cylinders, pH meter, sonicator, flow cytometer, 7300 Real-Time PCR System, etc.
Internal Number: 339351
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.