Essential and Other Functions: 60% Maintenance and Analysis of Human Cells: Characterize DNA repair deficiencies in human knockout cell lines. Complement knockout cell lines with the respective wild type and mutant genes. Analyze cells using survival assays, DNA repair assays, DNA fiber assays (immunofluorescence microscopy) and protein interaction studies.
30% Establish New Laboratory Protocols: Develop in vitro nucleosome assembly method for the laboratory and perform in vitro assembly assays with recombinant WT and mutant proteins.
10% Additional lab duties: Maintain human cell stocks and liquid nitrogen tanks. Perform general lab duties such as autoclaving, preparing gel running and transfer buffers, pouring agarose plates. Participate in supply ordering Present results in a weekly lab meeting and at a joint meeting with other laboratories.
Summary of Position: Responsibilities include: (1) performing duties that benefit the entire laboratory, such as autoclaving, preparing buffers and ordering; (2) working independently on a dedicated project after initial training by a postdoctoral fellow. The goal of the chosen project is to functionally characterize several human knockout cell lines that have deficiencies in DNA repair factors. The proposed work requires a variety of different techniques including human cell culture, cell infections, protein and chromatin immunoprecipitation and western blotting as well as functional DNA damage response assays. In addition, new techniques will be developed for the laboratory.
The University of Minnesota offers a comprehensive benefits package including:
Competitive wages, paid holidays, vacation and sick leave
Low cost medical, dental, and pharmacy plans
Health care and dependent daycare flexible spending accounts
Excellent retirement plans with employer match
Disability and employer paid life insurance
Wellbeing program with reduced insurance premiums
Tuition reimbursement opportunities covering 75%-100% of eligible tuition
Essential Qualifications: BA/BS with a specialization in a natural science discipline with at least 1 year of experience or a combination of related education and work experience to equal five years; experience comparing, verifying and reconciling data to help department achieve overall objectives. Solid experience with mammalian cell culture, cloning and cell survival assays is absolutely essential.
All required qualifications must be documented on application materials.
Preferred Qualifications: Previous experience with protein biology (western blotting, immunoprecipitations) and immunofluorescence microscopy is preferable but not necessary. Additional experience with chromatin biology assays would be a significant plus.
Internal Number: 339228
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.