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Researcher 1
Essential and Other Functions:40 % Molecular biologyDesign primers for PCR and run PCR, purify RNA and genomic DNA, construct plasmid vectors, prepare plasmids with miniprep and midiprep, prepare lentivirus and retrovirus, and analyze DNA sequences.30 % Cell biologyCulture of stem cells, induce differentiation of stem cells, transfect plasmid vectors, stain cells with antibodies, knock down or knock out genes with shRNA and CRISPR, and sort cells with a FACS.20 % Mouse workMaintain mouse colonies, do genotyping, and analyze histology sections.10 % General lab maintenanceMake solutions and gels for electrophoresis, organize reagents and chemicals, write and organize lab manuals, and prepare paperwork for animal license and
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