Essential and Other Functions: 40 % Molecular biology Design primers for PCR and run PCR, purify RNA and genomic DNA, construct plasmid vectors, prepare plasmids with miniprep and midiprep, prepare lentivirus and retrovirus, and analyze DNA sequences.
30 % Cell biology Culture of stem cells, induce differentiation of stem cells, transfect plasmid vectors, stain cells with antibodies, knock down or knock out genes with shRNA and CRISPR, and sort cells with a FACS.
20 % Mouse work Maintain mouse colonies, do genotyping, and analyze histology sections.
10 % General lab maintenance Make solutions and gels for electrophoresis, organize reagents and chemicals, write and organize lab manuals, and prepare paperwork for animal license and chemical license.
Summary of Position: Perform experiments to generate publishable data in stem cell biology and regenerative medicine. Main experiments include maintenance of mouse colonies, cloning of genes into plasmid vectors, PCR, bacterial culture, electrophoresis of DNA, fluorescence microscopy, and preparation of histology sections. Lab maintenance works include making solutions, inventory check of reagents, and maintenance of equipment. This position is suitable for those who have a substantial amount of experience in gene cloning and cell culture, allowing them to work independently with little supervision by the principal investigator.
The University of Minnesota offers a comprehensive benefits package including:
Competitive wages, paid holidays, vacation and sick leave
Low cost medical, dental, and pharmacy plans
Health care and dependent daycare flexible spending accounts
Excellent retirement plans with employer match
Disability and employer paid life insurance
Wellbeing program with reduced insurance premiums
Tuition reimbursement opportunities covering 75%-100% of eligible tuition
Student loan forgiveness opportunity
Opportunities for growth and promotion
Employee Assistance Program
For more information regarding benefits:
Researcher 1 is in the Civil Service Employment Group:
Required Qualifications: Bachelor's degree in life science with basic knowledge in genetics, biochemistry and molecular biology or a combination of the previous related education and work experience to equal four years in total. The ability to design and construct plasmid vectors and analyze DNA sequences with web-based programs, such as BLAST and UCSC genome browser.
Preferred Qualifications: 1-3 years of experience as a full-time lab technician. The preferred candidate will have good writing skills to prepare paperwork (lab safety report and animal license) without supervision. The ability to accurately record experiments in lab notebooks.
Internal Number: 337594
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.