Essential and Other Functions: 70 % Conduct Experiments (essential) Carry out various laboratory procedures and experiments that involve standard molecular biology techniques, including site-directed mutagenesis, SDS gel electrophoresis and western blotting. Generate strains of social amoeba, perform transformations and conduct a variety of phenotypic assays measuring cellular functions such as chemotaxis, adhesion, multicellular development. Conduct biochemical and biophysical analysis of protein domains (including CD spectroscopy, protein cross-linking and controlled proteolysis, mass spec, binding assays). Design and generate protein expression plasmids for testing models of protein function.
10 % Record and Analyze Data (essential) Record results, both manually and using custom image analysis software; analyze motility parameters using custom software as well as analyze DNA and protein sequences using Lasergene software. Prepare weekly summaries of results in PowerPoint presentations, summarizing results in charts and simple picture galleries.
10 % Operate Lab Equipment (essential) Operate routine lab equipment such as balances, pH meters, Coulter counter, centrifuges, thermal cycler and electroporator. Collect images using phase/DIC microscope and spinning disc confocal microscope.
5 % Laboratory Organization (essential) Store critical laboratory reagents, re-organize stocks in freezers and update computer logs, databases and protocols.
5 % Prepare Reagents (secondary) Prepare standard solutions or media according to prescribed formula.
Summary of Position: The Researcher 1 will provide critical support for the laboratory by assisting with cell biology and biochemistry experiments and analyzing data. They will be responsible for cell maintenance, performing routine molecular biology experiments, protein analysis, organizing stored materials (plasmids and strains) as well as contribute to general laboratory maintenance.
The University of Minnesota offers a comprehensive benefits package including:
Competitive wages, paid holidays, vacation and sick leave
Low cost medical, dental, and pharmacy plans
Health care and dependent daycare flexible spending accounts
Excellent retirement plans with employer match
Disability and employer paid life insurance
Wellbeing program with reduced insurance premiums
Tuition reimbursement opportunities covering 75%-100% of eligible tuition
Student loan forgiveness opportunity
Opportunities for growth and promotion
Employee Assistance Program
Researcher 1 is in the Civil Service Employment Group:
All required qualifications must be documented on application materials.
Required Qualifications: BA or BS in basic science (biology, microbiology or biochemistry only) or coursework in cell biology, molecular biology, biochemistry, biochemistry lab, tissue culture, protein engineering and microbiology, and a minimum of two semesters of independent research experience (beyond standard lab courses) that includes performing cell-based assays and data analysis. Combined education and experience must equal at least four years.
Preferred Qualifications: Experienced in quantitative light microscopy and programming with R, MatLab or equivalent. Familiarity with or experience in molecular biology techniques (cloning, Gibson Assembly, Golden Gate Assembly), cell culture and protein expression and analysis.
Internal Number: 337318
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.