A postdoctoral positions is available in the new laboratory of Dr. Alon Herschhorn at the Division of Infectious Diseases and International Medicine, Department of Medicine, University of Minnesota.
We are a new and energetic research group that works on exciting directions to understand and target HIV-1 host interactions at the molecular and cellular levels. We use interdisciplinary approaches at the interface of synthetic and molecular virology, immunology and cell biology to gain new insights into complex biological processes, with the aim of translating these insights into novel therapies and vaccines to treat and prevent viral infections.
Job Duties 70% - Research Primary duties and responsibilities will include, but are not limited to, studies of virus-host interactions using HIV-1 as a system model. Projects include analyzing HIV-1 interactions with neutralizing antibodies using viral assays, PCR amplification of HIV-1 genes from samples of infected individuals, development and application of new system for monitoring viral active and latent replication in primary cells, building new genetic circuits to investigate molecular interactions between viruses and the host, and studying the adaptation of HIV-1 to inhibitors and the environment.
The successful candidate will work in BSL2 and BSL2+ environment and build and use viral reporter systems to study the interactions of viruses with the cellular machinery. The research work involves RNA-seq and transcriptomic analysis using state-of-the-art instrument for single cell analysis, next generation sequencing, sort of infected cells using flow cytometry, and different molecular biology techniques to clone or modify genes.
The work also involves interaction with other lab member(s), participation in lab meeting, and supervision of undergraduate students.
30% - Data analysis, presentations, and manuscript preparation Analysis of experimental results. The successful candidate will use different computer programs to analyze next generation sequencing results (big data) and to associate cellular genes with viral infection. Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation.
Development of research directions according to the main project aims and the ongoing experiments
Preparation of figures and manuscripts based on the results
Reading literature related to the research projects and developing collaborations with other research groups
Preparation of data for presentations
The University of Minnesota offers a comprehensive benefits package including:
Competitive wages, paid holidays, vacation and sick leave
Low cost medical, dental, and pharmacy plans
Health care and dependent daycare flexible spending accounts
Excellent retirement plans with employer match
Disability and employer paid life insurance
Wellbeing program with reduced insurance premiums
Tuition reimbursement opportunities covering 75%-100% of eligible tuition
Required Qualifications - A Ph.D. (or equivalent) degree in virology, microbiology, cell biology, immunology or a related field - Advanced knowledge in molecular and cellular biology techniques. Experience in immunological methods such as ELISA, antibody characterization, and western blot; high proficiency in cloning and tissue culture techniques. - Excellent written and verbal communication and organization skills with proficient scientific record keeping - Proficiency in Microsoft Office applications including Word, Excel, and Power Point - Demonstrated ability to interact with other group members and oversee undergraduate work - Ability to work independently, pay attention to details, solve problems, work under pressure and manage multiple tasks - Experience and ability to analyze scientific data, present the research results and prepare manuscripts for publication. - Enthusiastic to work in new lab studying virus host interactions; team player, proactive, open-minded, creative and willing to learn
Preferred Qualifications - Experience in work with bacteria, human cells/tissues and viruses, including HIV-1, in a BL2+/BSL3 facility - Previous work with replication competent and single round HIV-1 assays, and adaptation/evolution in vitro experiments. - High proficiency in tissue culture work with different cell lines, isolation of RNA, analysis of gene expression using microarrays or RNA-seq. - Previous experience with performing and analyzing single-cell RNA-seq - Knowledge of and experience with isolation and maintenance of primary cells: Peripheral Blood Mononuclear Cells (PBMC) and CD4 T cells from blood - Experience with flow cytometry techniques and multi-parameter analysis - Proficiency in bioinformatic tools and experience with big data analysis of next generation sequencing results. - Experience with the analysis and validation of virus - cellular factors interaction at the molecular and cellular levels - Experience with bioengeening of lentiviral vectors and building new genetic circuits. - Experience with animal models and humanized mouse
Internal Number: 329071
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.